S-11 used in the laboratory activity laboratory flow chart s-13 used in the laboratory activity post-lab report s-15 used in the laboratory activity. To prepare competent cells and conduct transformation with plasmid dna • to confirm the background sections before you set foot in the lab we will be and transformation procedure c purification of pglo plasmid dna from bacteria.
Free essay: transformation lab report introduction transformation is the transfers of virulence from one cell to another, through the. To begin the experiment, we took two microcentrifuge tubes and labeled them + pglo and -pglo next, taking a micropipette with a clean tip. Justin nguyen 2/8/2016 period 7 bacterial transformation study abstract in the study below, our lab group will find out if it is possible to transform bacteria and. In this lab you will perform a procedure known as genetic transformation bio- rad's unique pglo plasmid encodes the gene for gfp and a gene for transformation efficiency is 1,000 bacteria/µg of dna, they often report this number as.
Bacterial transformation is the process by which a bacterium takes up and expresses foreign genetic material (dna), thus acquiring a new trait(s) in this lab , you. Ap lab #6: pglo transformation lab - free download as word doc (doc / docx ), pdf file (pdf) or read online for free. View lab report - pglo transformation lab from biology 162 at university of washington ap lab 8: pglo transformation lab introduction hypothesis: if we.
Lab report by: sonja silva date: 10/31/11 group members: janalee we transformed it with a pglo plasmid containing the gene for gfp and. Apbio- pglo lab report - download as word doc (doc / docx), pdf file (pdf) purpose: the purpose of this lab was to learn about genetic transformation by. Nicholas mack3 february 2016 pglo transformation lab report introduction: the purpose of this lab was to observe the effects o.
Bio 107 lab # 10: summary & study guide for day i, ii & iii labs you transformed e coli with pglo plasmid that contains gfp gene, arabinose c gene and.
A student researched experiment about a pglo lab only transformed bacteria that contain the plasmid and express beta-lactamase can.
The organism should grow vigorously in the lab environment, but genetically transformed cells have taken up the pglo plasmid which expresses transformation efficiency is 1,000 bacteria/ug of dna, they often report this number as. Core content subjects into a single lab connect concepts with techniques and put them into context with real-world scenarios pglo bacterial transformation.
By screening cells transformed with mutagenized pglo with and gr using bl2seq and include the printouts of your results with this report. [APSNIP--]